Translational_Unit

Part:BBa_K3803015:Design

Designed by: Shiyu Yu   Group: iGEM21_Jiangnan_China   (2021-09-02)


XK translational unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2860
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2860
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2860
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon of XK from Scheffersomyces stipitis had been optimized for better expression in S. cerevisiae


Source

The promoter and terminator is from S. cerevisiae, and the coding gene of XK is from Scheffersomyces stipitis.

References